il-23 and il-27 levels in macrophages collected from peripheral blood of patients with healing vs non-healing form of cutaneous leishmaniasis

نویسندگان

s tolouei department of parasitology and mycology, school of medicine, isfahan university of medical sciences, isfahan, iran

k ghaedi department of biology, school of sciences, university of isfahan, department of cell and molecular biology, royan institute for animal biotechnology, acecr, isfahan, iran

a khamesipour center for research and training in skin diseases and leprosy, tehran university of medical sciences, tehran, iran

m akbari school of medicine, isfahan university of medical sciences, isfahan, iran

چکیده

background: in this study the level of il-23 and il-27 produced by macrophages derived from peri­pheral blood mononuclear cell culture collected from patients with healing or non-healing form of cutane­ous leishmaniasis lesion were compared before and after treatment with live leishmania to explore whether il-23 or il-27 plays any role in healing process of cutaneous lesions induced by l. major . methods: twenty patients resident in isfahan province, with healing or non-healing form of cutaneous leishmaniasis lesion caused by leishmania major participated in this study. in vitro productions of il-23 and il-27 by peripheral blood derived macrophages, before and after stimulation with live l. major (mrho/ir/75/er) promastigotes were evaluated using elisa method . patient with healing form of le­sion received no treatment and patient with non-healing form of lesion received at least 2 courses of glucan­time. results: the mean production of il-23 and il-27 from macrophages of patients with healing form of lesion was significantly higher than patients with non-healing form of lesion. the levels of il-23 and il-27 in culture supernatants before and after stimulation in healing form of cl was significantly higher than non- healing form of cl ( p < 0.001). conclusion: il-23 and il-27 might play a role in human leishmaniasis and further studies are needed to understand the role of il-23 and il-27 in leishmaniasis.

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منابع مشابه

IL-23 and IL-27 Levels in Macrophages Collected from Peripheral Blood of Patients with Healing Vs Non-Healing Form of Cutaneous Leishmaniasis

BACKGROUND In this study the level of IL-23 and IL-27 produced by macrophages derived from peripheral blood mononuclear cell culture collected from patients with healing or non-healing form of cutaneous leishmaniasis lesion were compared before and after treatment with live Leishmania to explore whether IL-23 or IL-27 plays any role in healing process of cutaneous lesions induced by L. major. ...

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عنوان ژورنال:
iranian journal of parasitology

جلد ۷، شماره ۱، صفحات ۱۸-۲۵

کلمات کلیدی
[ ' i l ' , 2 3 , ' i l ' , 2 7 , ' m a c r o p h a g e s ' , ' p r o m a s t i g o t e ' , ' c u t a n e o u s l e i s h m a n i a s i s ' ]

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